By WWII, scientists had already begun looking at alternative gelling substances for routine use in bacteriology, but concluded that agar was still better as it is both firmer and easier to handle. Today, some specialized microbiology applications use the colloid carrageenan (extracted from red seaweed Chondrus crispus, or “Irish Moss”), a more transparent and less auto-fluorescent alternative to agar (agar emits its own background fluorescence when excited by light). However, for routine bacteriological use, carrageenan is more difficult to dissolve, requires higher concentrations, can degrade at high temperatures, and forms weaker gels, which may result in puncturing its surface during the plating of cells.
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The identification of ‘boosters’ that drive gene overexpression directly in a CAR construct provides a simple and scalable strategy for developing effective CAR-NK cell therapies for solid tumours.
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The 386 microcode sequencer has a one-cycle pipeline delay: when a jump or RNI (run next instruction) is decoded, the micro-instruction immediately after it has already been fetched and will execute before the jump takes effect. This "delay slot" is a basic property of the sequencer, and the microcode is written to fill it with useful work rather than waste a cycle on a bubble. The examples in the PTSAV section above show this: at 582/5AE, the micro-instruction after LCALL executes before the subroutine begins.
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